大熊猫西氏贝蛔虫BsABF基因的原核表达及重组蛋白抗菌活性初步分析

doi:10.11843/j.issn.0366-6964.2019.01.018

摘要/Abstract

摘要：

本研究旨在探究大熊猫西氏贝蛔虫一个抗菌肽分子的生物学特性及其重组蛋白rBsABF在体外的抗菌活性。对西氏贝蛔虫抗菌肽BsABF基因进行克隆、原核表达及重组蛋白rBsABF的纯化，通过免疫印迹与荧光免疫组化分析重组蛋白rBsABF免疫反应性及BsABF在虫体中的分布；采用抗菌试验对重组蛋白rBsABF的抗菌活性进行了初步评价。研究结果显示，BsABF基因ORF全长276 bp，编码蛋白分子大小为10.01 ku，带有N端信号肽，与猪蛔虫同源基因序列相似性为94%，主要分布于虫体体壁、肠道与虫卵，重组蛋白rBsABF具有良好的免疫反应性。抗菌试验结果显示该重组蛋白对大熊猫源粪肠球菌及大肠杆菌具有一定的抗菌活性。首次鉴定了大熊猫西氏贝蛔虫BsABF基因，抑菌试验表明重组蛋白rBsABF具有抗菌活性，为今后深入研究该蛋白的功能特性奠定了基础。

Abstract:

This experiment was conducted to explore the characterization and the antibacterial activity of an antibacterial factor in Baylisascaris schroederi. The gene BsABF was cloned and expressed with prokaryotic expression system. Western blot and immunofluorescence histochemical staining were used to determine the immunological characteristics and the location in the parasite. The antibacterial activity was evaluated by the preliminary antibacterial experiments. Results were as follows:Open reading frame of BsABF gene (276 bp) encoded a 10.01 kDa protein with an N-terminal signal peptide while sharing 94% identity with Ascaris suum. The protein is mainly distributed in hypodermis, intestinal and eggs. The prokaryotic expression of rBsABF had a good immunoreactivity. Meanwhile the antibacterial assessment revealed that the recombinant protein has the antibacterial activity towards Enterococcus faecalis and Escherichia coli in giant pandas. In this study, we have first identified the BsABF gene, and rBsABF does have the antibacterial activity, and it lays the foundation for further study on the functional properties of BsABF in the future.

中图分类号:

S852.731

吴怡然, 古小彬, 谢跃, 杨光友. 大熊猫西氏贝蛔虫BsABF基因的原核表达及重组蛋白抗菌活性初步分析[J]. 畜牧兽医学报, 2019, 50(1): 151-158.

WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.

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